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Does the NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent work well for GC-rich DNA and AT-rich DNA?.

How much starting material do I need to use when preparing libraries using the NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent?.

What type of starting material can be used with NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent?.

What sequencing platform can I use the NEBNext Fast DNA Library Prep Sets for Ion Torrent reagents for?.

Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page This product is related to the following categories: Library Preparation for Ion Torrent This product can be used in the following applications: DNA Sequencing The lots provided are managed separately and qualified by additional functional validation. coli DNA was fragmented and end repaired for 20 minutes at 25☌, followed by 10 minutes at 70☌.įigure 2: Final Library size distribution using AMPure XP Bead Size Selection.įigure 3: Final Library Size distribution using E-Gel Size Selection. Read length histogram from 200 bp run.įigure 1: Relative size distribution of Fragmented End Repaired DNA as seen using the Bioanalyzer ® 2100 (Agilent Technologies, Inc.).ġ µg of E. 72 Mb of data was generated, with an average genome coverage of 15.5 X, from approximately 0.5 Million 200 bp reads.

coli (K12 MG1655 strain) genomic DNA using NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent. Representative Results from Libraries Constructed Using NEBNext for Ion TorrentĠ.5 µg of DNA from 3 different genomes with varying GC content were used to construct 200 bp and 400 bp libraries using the NEBNext Fast DNA Fragmentation and Library Prep Set for Ion Torrent, analyzed by the Agilent® Bioanalyzer®.Ī typical Ion Torrent run report for libraries made from E. If mechanical shearing of DNA is preferred, a version of the kit without enzymatic fragmentation reagents is available.ĭNA Library Preparation Workflow for Ion Torrent For multiplexed libraries, the Ion Xpress™ Barcode Adapters from Thermo Fisher Scientific can be used. Adaptors and primers for singleplex libraries are supplied in the kits. The kit is compatible with input amounts from 10 ng - 1µg, with a workflow time of under 2 hours and ~12 min. This enables efficient and tunable DNA shearing and blunting with minimized sample loss before performing adaptor ligation and high-fidelity library amplification. The NEBNext ® Fast DNA Fragmentation & Library Prep Set for Ion Torrent ® includes a master mix of reagents for enzymatic DNA fragmentation combined with end repair.